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Custom cDNA Library Construction Services

DTI offers high-quality customized cDNA libraries for the specific needs of fellow researchers. Our staff has over ten years of experience in constructing cDNA and genomic libraries from a variety of cells and tissues such as:

Human, Mouse, Rat, Monkey, Plant, Algae, Bacteria, Fungi, Yeast, Fruit Fly, Protozoa, Fish, etc.

DTI’s customized cDNA libraries have the following advantages:

  • Rigorous mRNA QC steps to test ribosomal RNA and genomic DNA contaminations
  • High quality size-fractionated large insert cDNA library (average insert size 1-2 kb)
  • Choice of vector for library construction
  • Low vector background and high percentage of recombinants (over 90% recombinant)
  • Large number of primary clones (3-6 x 106 for standard library and 1-2 x 106 for normalized or subtracted libraries)
  • Library transformation into T1 phage resistant E. coli

Standard cDNA Library Construction

  • We guarantee to provide 3-5x106 primary clones
  • Average insert size is least 1-1.5 kb and Over 90% recombinants
  • The libraries can be made directionally and in the choice of vector for your special needs
  • Two size selected cDNA libraries are provided (0.5 - 2 kb and 2+ Kb)
  • For quality control purposes we test 10-15 random clones by PCR or restriction digestion
  • The library is provided in glycerol or DMSO

Normalized cDNA Libraries

  • Normalization is carried out at various Cot levels (Cot10, Cot50 or Cot500) according to relative abundance to genes that needs to be normalized
  • Percent Normalization is tested by:
    • Relative PCR for house keeping genes and low abundant genes
    • Colony/plaque hybridization of 3,000 – 30,000 clones before and after normalization
    • DNA sequencing of 96 random cDNA clones
  • We guarantee to provide 1x106 primary clones
  • Two size selected cDNA libraries are provided (0.5 - 2 kb and 2+ Kb)
  • Average inserts size of at least 1 -1.5 kb and over 90% recombinants
  • The libraries can be made directionally and in the choice of vector for your specific needs

Subtracted cDNA Libraries

  • Subtraction is carried out according to relative abundance to genes that needs to be subtracted
  • Percent Subtraction are tested by:
    • Relative PCR for house keeping genes and low abundant genes
    • Colony/plaque hybridization of 3,000 – 30,000 clones before and after subtraction
    • DNA sequencing of 96 random cDNA clones
  • We guarantee to provide 1x106 primary clones
  • Two size selected cDNA libraries are provided (0.5 - 2 kb and 2+ Kb)
  • Average inserts size of at least 1 -1.5 kb and over 90% recombinants
  • The libraries can be made directionally and in the choice of vector for your specific needs
Ordering Information
Item Catalog Number Price
Custom Standard cDNA Construction Services7110 $ Please Inquire
Custom Normalized cDNA Library Construction Services 7111 $ Please Inquire
Custom Subtracted cDNA Library Construction Services 7112 $ Please Inquire

For your customized needs please contact our technical services department via telephone or email us at info@dnati.com.

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Gaithersburg, MD 20877, USA | Tel: 855-677-0686, 301-337-6648 | info@dnati.com