• Cloning Kits and Reagents
• Electrophoresis Kits and Reagents
• Microarray Related Kits
• DNA/ RNA Isolation Kits
• cDNA Synthesis Kits
• DNA Amplification Kits
• Tissue ReadyLysates™
• Pre-Made Western Blots
• Immuno Detection Kits
• Enzymes
• Pure™ Reagents
• Essential Lab Supplies

• Plasmid DNA Manufacturing
• DNA/ RNA Isolation
• cDNA Library Construction
• Genomic Library Construction
• Genomic Library Screening Services
• Real Time PCR
• DNA Sequencing
• Oligo Synthesis
• Gene Cloning and Expression
• Recombinant Protein Purification

DTI offers high-quality customized cDNA libraries for the specific needs of fellow researchers. Our staff has over ten years of experience in constructing cDNA and genomic libraries from a variety of cells and tissues such as:

Human, Mouse, Rat, Monkey, Plant, Algae, Bacteria, Fungi, Yeast, Fruit Fly, Protozoa, Fish, etc.

• Standard cDNA Libraries
• Normalized cDNA Libraries
• Subtracted cDNA Libraries

Standard cDNA Library Construction

• We guarantee to provide 3-5x10⁶ primary clones
• Average insert size is least 1-1.5 kb and Over 90% recombinants
• The libraries can be made directionally and in the choice of vector for your special needs
• Two size selected cDNA libraries are provided (0.5 - 2 kb and 2+ Kb)
• For quality control purposes we test 10-15 random clones by PCR or restriction digestion
• The library is provided in glycerol or DMSO

Normalized cDNA Libraries

• Normalization is carried out at various Cot levels (Cot₁₀, Cot₅₀ or Cot₅₀₀) according to relative abundance to genes that needs to be normalized
• Percent Normalization is tested by:
  • Relative PCR for house keeping genes and low abundant genes
  • Colony/plaque hybridization of 3,000 – 30,000 clones before and after normalization
  • DNA sequencing of 96 random cDNA clones
• We guarantee to provide 1x10⁶ primary clones
• Two size selected cDNA libraries are provided (0.5 - 2 kb and 2+ Kb)
• Average inserts size of at least 1 -1.5 kb and over 90% recombinants
• The libraries can be made directionally and in the choice of vector for your specific needs

Subtracted cDNA Libraries

• Subtraction is carried out according to relative abundance to genes that needs to be subtracted
• Percent Subtraction are tested by:
  • Relative PCR for house keeping genes and low abundant genes
  • Colony/plaque hybridization of 3,000 – 30,000 clones before and after subtraction
  • DNA sequencing of 96 random cDNA clones
• We guarantee to provide 1x10⁶ primary clones
• Two size selected cDNA libraries are provided (0.5 - 2 kb and 2+ Kb)
• Average inserts size of at least 1 -1.5 kb and over 90% recombinants
• The libraries can be made directionally and in the choice of vector for your specific needs

For your customized needs please contact our technical services department via telephone or email us at info@dnati.com.