- Reagents for Detecting Quantity of Low, Moderate and High Expression mRNA Sequences
- Simple, and Efficient Analysis of Normalization and Subtraction Process
mRNA CopyDetect™ System analyzes the quality of cDNA synthesis, normalization
or subtraction process by analyzing gene copy; thus saving valuable time, research effort, and
expensive reagents on subsequent molecular manipulation studies. The kits provides the
following applications:
- Test the quality of cDNA synthesis & cDNA Library Construction
- Measure the purity of RNA and DNA during your isolation procedures
- Run parallel PCR reactions and get an idea about Quantitative RT-PCR
- Determine Comparative Gene Expression Profiles of your target gene by ‘Normalization’ with house keeping genes in a single tube
The kit provides reagents to detect expression levels of housekeeping genes
from highly abundant, moderately abundant and lower levels. Generally, highly abundant genes
exists at ~3,000 copies per cell, moderately abundant genes are expressed at 300-3,000 and
low abundant genes at <300 i.e. they express at 1%, 0.1%-1.0%, and <0.1% of total RNA. Our
mRNA CopyDetect™ Kit contains four sets of different RT-PCR primers that are used to amplify
human or mouse housekeeping genes.
- High Copy Genes
- Mitochondrial 16s RNA
- Cytoplasmic Beta Actin
- Elongation Factor EF-1
- Moderate Copy Genes
- Alfa Tubulin
- Beta Globin
- Phospholipase A2
- Low Copy Genes
Kit Components
- Gene Detect PCR Amplicon Sets (High, Medium or Low Copy)
- 10x DNA Amplification Buffer
- Taq DNA Polymerase
- 10x PCR dNTP Mix
- Nuclease Free Water
- Control cDNA Template
Storage Condition and Stability
mRNA Isolation System is stored at -20o C and stable for six months.
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