- Convert recessed 3’- and 5’- ends for cloning purposes
- All necessary reagents are provided with the kit
DTI’s DNA Blunting System provides reagents for converting recessed 3’- and 5’- ends
for cloning purposes. The reagents provide the researcher a quick, reliable and convenient
system for shotgun cloning of DNA fragments or PCR products into plasmid, cosmid or lambda
phage vectors.
Applications
- Rapid cloning of DNA for gene expression purposes
- Construction of recombinant plasmids for shot gun libraries and DNA sequencing applications
Kit Components
- DNA Polymerase Cocktail, 50 µl
- 10x Blunting Reaction Buffer, 100 µl
- 10x dNTP Mix, 100 µl
- Nuclease Free Water, 500 µl
- Instruction Manual
Storage Condition and Stability
Kit components are stored at -20o C and stable for 3-6 month.
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